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Developing Yeast as a Cellular-based Neurotransmitter Fluorescently Engineered Reporter for Acetylcholine

William Valentine McMichael

Name: William Valentine McMichael
Major: Neurobiology
Advisors: Dr. William Morgan, Dr. Seth Kelly

The ability to detect neurotransmitter release and concentration at the synapse is of particular importance in understanding the pathology of neuronal disorders. Cellular based neurotransmitter fluorescently engineered reporters (CNiFERs) are a recently developed tool to image neurotransmitter release with high temporal and spatial resolution. Developing these reporters in yeast may provide a cheaper and more flexible alternative to using mammalian cell lines for reporter expression. Measurements of plasmids from two transformations in E. Coli were confirmed, and transformation of yeast was successful. Further work is needed to test the fluorescent activity of the engineered reporter. Some literature suggests that yeast may not be suitable for neurotransmitter sensing. Future work may use the yeast strain for investigating affinities and kinetics of potential ligands.

Posted in Comments Enabled, Independent Study, Symposium 2022.


One response to “Developing Yeast as a Cellular-based Neurotransmitter Fluorescently Engineered Reporter for Acetylcholine”

  1. Maha Rashid says:

    Interesting IS! Why chose ACh?